Journal: Cell reports

Article Title: Chronic Corticosterone Elevation Suppresses Adult Hippocampal Neurogenesis by Hyperphosphorylating Huntingtin.

doi: 10.1016/j.celrep.2020.107865

Figure Lengend Snippet: Figure 4. Phosphorylation of HTT at S1181/S1201 Influences Hippocampal Neurogenesis (A–C) Cells counts were performed in the DG of vehicle- and CORT-treated WT and HdhS1181A/S1201A mice (at least 4 for animals per condition) to quantify proliferation (Ki67+ cells, A), survival (BrdU+ cells, B), and immature neurons (DCX+ cells, C). *p < 0.05, **p < 0.01, ***p < 0.001. (D) Representative images show BrdU+ surviving cells (cyan) expressing (or not) the terminal marker for granule cell differentiation calbindin (magenta) on DG sections of WT and HdhS1181A/S1201A mice treated with vehicle or CORT. (E) The histogram shows the quantification of the number of BrdU+ and calbindin+ cells (3 animals per condition). *p <0.05, **p < 0.01. (F and G) Dendritic length (F) and complexity (G) were evaluated in both genotypes and treatment conditions (3 animals per condition). (H) Representative drawings of dendrites of DCX-positive neurons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 comparing the HdhS1181A/S1201A vehicle group with the WT vehicle group, $p < 0.05, $$p < 0.01, $$$p < 0.001, $$$$p < 0.0001 comparing the HdhS1181A/S1201A -CORT group with the WT CORT group. Error bars, SEM. See also Figure S2.

Article Snippet: Slices were rinsed and blocked in 3% normal donkey serum in PBS containing 0.3% Triton X-100 for 1h at room temperature then incubated overnight at room temperature in 1:100 of mouse anti-BrdU antibody (#347580, BDBiosciences) and 1:500 rabbit anti-calbindin antibody (#13176, Cell Signaling Technologies).

Techniques: Phospho-proteomics, Expressing, Marker, Cell Differentiation

Journal: Medicine

Article Title: Loss of MAPK Pathway Activation in Post-Mitotic Retinal Cells as Mechanism in MEK Inhibition-Related Retinopathy in Cancer Patients

doi: 10.1097/MD.0000000000003457

Figure Lengend Snippet: ERK activation in nonregenerative primary neuroretinal tissue. (A) Binimetinib reduced ERK activity (P-ERK) in primary neuroretinal tissue. MEK inhibition had no effect on the expression of calbindin, vinculin, and total ERK. (B) In all 7 neuroretinas, a reduction in ERK activity was observed after treatment with binimetinib. The differences in activity after treatment with binimetinib concentrations of 25 and 125 nM, when comparing to 0 nM, were significant ( P = 0.004 and P < 0.001, respectively). Between the binimetinib concentrations, no significant difference was seen in total ERK expression. The integrated intensities of both ERK and total ERK were corrected for vinculin ([{p}ERK/vinculin] × 100). Bars represent mean with SEM. ∗ P < 0.05 compared to control (0 nM), and ∗∗ P > 0.05 compared to control (0 nM). ERK = extracellular signal-regulated kinase, MEK = mitogen-activated protein kinase kinase, SEM = standard error of the mean.

Article Snippet: The primary antibodies used included mouse-anti-phospho-p44/42 ERK antibody (Sigma Aldrich), rabbit-anti-p44/42 ERK antibody (Cell Signalling Technologies, Danvers, MA), mouse-anti-vinculin antibody (Sigma Aldrich), and rabbit-anti-calbindin-D28K antibody (Cell Signalling Technologies).

Techniques: Activation Assay, Activity Assay, Inhibition, Expressing, Control

Journal: Cell reports

Article Title: Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST

doi: 10.1016/j.celrep.2019.03.072

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-calbindin D-28K monoclonal antibody (clone CB-955) , Acris Antibodies , Cat# AM08219SU-N; RRID: AB_1954252.

Techniques: Expressing, Plasmid Preparation, Recombinant, SYBR Green Assay, Reporter Assay, Isolation, Staining, Microarray, Clone Assay, Software

Journal: Frontiers in Neuroscience

Article Title: Dystrophin Is Required for the Proper Timing in Retinal Histogenesis: A Thorough Investigation on the mdx Mouse Model of Duchenne Muscular Dystrophy

doi: 10.3389/fnins.2020.00760

Figure Lengend Snippet: Primary antibodies used for immunofluorescence.

Article Snippet: Rabbit anti-calbindin (HC) , 1:1000 , Merck Millipore (Billerica, MA, United States).

Techniques: Immunofluorescence